- Countries/Regions
- United States of America
- Category
- Research
- Academic Field
- Medicine
- Related goals of SDGs
| Keyword |
Genetically modified mice |
Information on Niigata University
| Name of the professor/researcher |
Toshikuni Sasaoka |
| Position/Faculty |
Professor, Brain Research Institute |
Information on the Counterpart
| Countries/Regions |
United States of America |
| Faculty/Institution |
University of Illinois at Urbana-Champaign |
Detailed Information/Report on Activities
In collaboration with Prof. Sasaoka and Professors Sakimura and Abe, Dr. Yanyan Wang have made progress in generating the dopamine D2S conditional knockout (D2S cKO) mouse using the new RNA splicing and Cre-loxP recombination method. The D2S cKO is designed to express both D2L and D2S isoforms before Cre-loxP recombination and express only D2L after Cre-loxP recombination. Profs Sakimura and Abe have already obtained the targeted recombinant ES cells and generated progeny mice derived from ES cells. Prof. Sasaoka group is in the process of breeding these mice with recombinase-expressing mice to produce D2S cKO mice. It is expected that the D2S cKO mice will be obtained in 4-5 months.
In addition, we have established the condition and protocol for studying synaptic transmission in mouse striatal neurons using electrophysiological recordings in corticostriatal slices.
This collaborative research will enhance our understanding of the role of dopamine D1 receptor (D1R) and two isoforms of the dopamine D2 receptor (D2R) in the regulation of motor activity and cognitive functions and in dopaminergic-related neuropsychiatric disorders.
